FUW TRENDS IN SCIENCE & TECHNOLOGY JOURNAL
(A Peer Review Journal)
e–ISSN: 2408–5162; p–ISSN: 2048–5170
keywords: Cassava peel, Aspergillus niger, protein, cyanide, fermentation, livestock
Aspergillus niger ANL301 B-1 β-glucosidase was investigated on its effectiveness in optimizing protein contents and reduction in cyanide content of cassava peels. Freshly harvested cassava tubers peels were dried in oven at 70oC for 36 h and milled into fine particles using micro millers. Two sterilized conical flasks (250 ml), each containing 50 g of the cassava peel flour in triplicates were prepared. Thirty (30) ml of sterile basal medium was added into each flask and thoroughly mixed. The flasks and their contents were sterilized by placing in boiling water at 100oC in a GFL brand water bath for 5 min and in the autoclave at 121oC for 15 min. After cooling, one ml of spore suspension of A. niger was aseptically introduced into the first set of the flasks plugged with cotton wool and dried in the oven at 60oC for 24 h. The second set of flasks had no inoculums, were plugged with cotton wool and dried at room temperature. Results showed that fermentation of cassava peels by solid state fermentation using the A. niger significantly (p<0.05) enriches the protein content of the waste and drastically lowering the cyanide concentration The significant changes in crude protein and cyanide contents after fermentation suggests it could be used as a good source of protein in compounding animal feeds. These findings will facilitate the development of an improved method for enhancing the nutritional value of cassava peels and other waste products.
Anupama P & Pavindra A 2000. Value-added food: Single cell protein. Biotech. Advances, 18: 459- 479. Achinewhu SC, Barber LI & Ijeoma IO 1998. Physicochemical properties and garification (gari yield) of selected cassava cultivars in rivers state, Nigeria. Plant Foods for Human Nutr., 52(2): 133-140. Akinyele BJ & Agbro O 2007. Increasing the nutritional value of plantain wastes by the activities of fungi using the solid state fermentation technique. Res. J. Microbio., 2: 117-124. Balagopalan C 1996. Nutritional Improvement of Cassava Products Using Microbial Techniques for Animal Feeding. Monograph of the Central Tuber Research Institute, Kerala, India, p. 44. Bradford M 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248–254. Bhavsar K, Kumar J & Khire M 2011. High level phytase production by Aspergillus niger ncim 563 in solid state culture: Response surface optimization, up-scaling, and its partial Charact- erization. J. Ind. Microbio. & Biotech., 38: 1407–1417 Farinas CS, Farinas MM, Loyo AB, Junior PW, Tardioli VB & Neto S 2010. Courifinding stablecellulase and xylanase: Evaluation of the synergistic effect of ph and temperature. New Biotech., 27: 810–815. Fisher RA 1950. Statistical methods for research workers 11th edition oliver and boyd, London, p. 75. Ganiyu O 2005. Nutrient enrichment of cassava peels using a mixed culture of Saccharomyces cerevisae and Lactobacillus spp solid media fermentation techniques. Electronic J. Biotech., 9(1): 0717-3458. Hughes J, Carvalho FJP & Hughes MA 1994. Purification, characterization, and cloning of α-hydroxynitrilelyase from cassava (Manihotesculenta). Arch Biochembiophys, 311: 496–502. Iyayi EA 2004. Changes in the cellulose, sugar and crude protein contents of agro-industrial by-products fermented with Aspergillus niger, Aspergillus flavus and Penicillum sp. Afr. J. Biotechn., 3: 186-188. Lowry OH, Rosebrough NJ, Farr AL & Randall RJ 1995. Protein measurement with folin phenol reagent. J. Biol. Chem., 93: 112 Mitidieri A, Mitidieri AH, Martinelli A & Schrank MH 2006. Vainsteinenzymatic detergent formulation containing amylase from Aspergillus niger: A comparative study with commercial detergent formulations. Bioresource Techn., 97: 1217–1224. Morya VK, Morya S, Yadav E & Kim D 2012. Yadavinsilico characterization of alkaline proteases from different species of Aspergillus. Appli. Biochem. & Biotech., 166: 243–257. Jamal PM, Tompang MZ & AlamA D 2009. Optimization of media composition for the production of bioproteinfrom pineapple skins by liquid-state conversion. J. Appl. Sci., 9(17): 3104 – 3109. Nigam P & Singh D 1994. Solid state (substrate) fermentation systems and their applications in biotechnology. J. Basic microbiol., 34: 405-414. Ofuya CO, Nwanjuiba CJ 1990. Microbial degradation and utilization of cassava peel. World J. Microbio. Biotech., 6: 144-148. Oboh G & Akindahunsi AA 2003. Biochemical changes in cassava products (flour & gari) subjected to Saccharomyces cerevisae solid media fermentation. Food Chem., 82(4): 599 – 602. Obioha FS1972. Utilization of Cassava as Human Food. A literature review and research recommendations on cassava. Aid contract No. Csd/2497, 131
